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KMID : 0613820160260010109
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Journal of Life Science
2016 Volume.26 No. 1 p.109 ~ p.116
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Ulmus macrocarpa Hance Water Extract Improved Splenocytes Survival and NK Cell Cytotoxicity
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Lee Sung-Do
Kim Deok-Won
Lee In-Hwan
Lee Jong-Hwan
Hyun Sook-Kyung
Kang Kyung-Hwa
Hwang Hye-Jin
Kim Cheol-Min
Kim Byung-Woo
Chung Kyung-Tae
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Abstract
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Ulmi cortex is the elm bark or root bark of Ulmus macrocarpa Hance and has been used as an ingredient of traditional medicine for anti-inflammatory, analgesic, anti-cancer and wound healing on both the East and the West. This study investigated whether the Ulmus macrocarpa Hance Water extract (UMWE) has the in vivo and in vitro immune activating effect. Animals were orally administrated for 14 days as follows: no treat group with distilled water, cyclophosphamide (CY) group with 120 mg/kg of CY, UMWE 100+CY group with 100 ㎎/㎏ of UMWE and 120 mg/kg of CY, UMWE 200+CY group with 200 ㎎/㎏ of UMWE and 120 mg/kg of CY, UMWE 100 group with 100 ㎎/㎏ of UMWE and UMWE 200 group with 200 ㎎/㎏ of UMWE. The immunosuppressive drug CY was intraperitoneally injected to induce immune suppression. Spleen indices showed small changes in CY injected groups but splenocyte indices showed greater decrease in the same groups. However, UMWE appeared to relieve CY's immunosuppression. UMWE also delayed in vitro splenocyte death increasing its longevity. These data obtained by MTT assay and 7-amino-actinomycin D which stains preferentially dead than live cells. UMWE alone did not show cytotoxicity based on its apoptototic effect on splenocytes in vitro and in vivo. Splenic NK cell activity was maintained by UMWE under the presence of CY in vitro. The data indicated that UMWE protects splenocytes from the immunosuppressive drug CY under in vitro and in vivo conditions.
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KEYWORD
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Immune function, NK cell activity, splenocyte survival, cyclophosphamide, Ulmus macrocarpa Hance
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